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Part:BBa_K2150020:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pT7+pTet+RBS+GFP+RBS+T7RNAP+DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752


Design Notes

By comparing fluorescence intensity of this part and BBa_K2150018(pT7+pTet+RBS+GFP+DT), the positive feedback effect of T7RNAP-pT7 system can be measured. This part is for logic verification.


Source

pT7:PCR amplified from BBa_K525998,T7RNAP:PCR amplified from genome of BL21.

pTet from BBa_R0040,RBS from BBa_B0034,GFP from BBa_E0040,DT from BBa_B0015

References